Combining Single-Cell and Spatial Transcriptomics Uncovers Glycolytic Diversity and NEK6-Driven Advancement in Colorectal Cancer - Takeaways - MDSpire

Combining Single-Cell and Spatial Transcriptomics Uncovers Glycolytic Diversity and NEK6-Driven Advancement in Colorectal Cancer

  • By

  • Fengming Yang

  • Yinuo Bian

  • Yuqi Jin

  • Yinuo Tan

  • Tianhao Lao

  • Jie Yang

  • Hua Chen

  • April 28, 2026

  • 0 min

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  • 1

    This study integrates single-cell RNA sequencing and spatial transcriptomics to explore glycolytic diversity in colorectal cancer.

  • 2

    Three distinct malignant subtypes were identified, with Glycolysis-C1 exhibiting a high-glycolytic state and acting as a signaling hub.

  • 3

    Glycolysis-C1 cells remodel the immune microenvironment through MIF-CD44 signaling, influencing macrophage and B cell interactions.

  • 4

    NEK6 was identified as a candidate gene linked to colorectal cancer risk, with high expression correlating to poor prognosis.

  • 5

    Knockdown of NEK6 in CRC cell lines significantly reduced proliferation, glycolysis, and M2 macrophage polarization.

Original Source(s)

Combining Single-Cell and Spatial Transcriptomics Uncovers Glycolytic Diversity and NEK6-Driven Advancement in Colorectal Cancer

  • by Fengming Yang, Yinuo Bian, Yuqi Jin, Yinuo Tan, Tianhao Lao, Jie Yang, Hua Chen

  • April 28, 2026

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