One Blood Spot, Two Omics Readouts

Single sample supports separate metabolite and lipid profiling in positive- and negative-ion modes

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A new protocol allows simultaneous extraction of metabolites and lipids from a single dried blood spot for two omics analyses.
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The method utilizes a modified Folch extraction with a 2:1 chloroform and methanol mixture to separate metabolites and lipids.
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The workflow includes reversed-phase liquid chromatography-tandem mass spectrometry for separate analysis of metabolites and lipids.
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Data processing employs open-source tools Mzmine for metabolite features and LipidMatch for lipid data.
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Results are semiquantitative, allowing comparison of peak heights or areas, but absolute concentrations require further validation.

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