Quantitative phosphoproteomic profiling of CCL5/CCR5 signaling cascade in melanoma cells

1

The CCL5/CCR5 axis is crucial for tumor progression and metastasis in melanoma.
2

Quantitative phosphoproteomics identified 393 regulated phosphosites across 315 phosphoproteins after CCL5 stimulation.
3

Temporal analysis showed modulation of 256, 134, and 83 phosphosites at 5, 10, and 30 minutes post-CCL5 treatment.
4

Comparative phosphoproteomics revealed 52 phosphoproteins with distinct temporal dynamics between CCR5 knockout and wild type cells.
5

Gene Ontology analysis indicated CCL5/CCR5 involvement in various biological processes, notably cell cycle regulation.

Frontiers In Oncology

by Xie, Lifen , Zhu, Tianhui , He, An , Wu, Qin , Zeng, Jinfeng , Huang, Liqin , Zhang, Ling , Liu, Jie
June 22, 2026

1